FastQCFastQC Report
Mon 6 Jan 2020
NeoHDF_BC0_1_Arid1a_Pan3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameNeoHDF_BC0_1_Arid1a_Pan3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032797
Sequences flagged as poor quality0
Sequence length29
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTGATTAGAAACGAGGCTGGTTTGGG1824423.0241693861073062No Hit
TGGTTTTATCACATTCTGAATTGAACGTT117630.1949841839531481No Hit
AGGCTCTGGTTATCTACTGAAATGCTGAA117410.194619510651527No Hit
CCCGGGCCAGAAGGAGCTGCAAGACGGGG99350.16468314780026577No Hit
TGCAAAGCATTCCACTCCATTACTTCTTT93270.15460490382819114No Hit
CTCTCTTCAATTTACTGTGTGTGCTTTCA88510.14671469966584325No Hit
AGCAAACAAACTAGCCTGACAGAACTAGC86980.14417856261365997No Hit
ATTCACAAAAACACCCTCCTCCCGGCTCT83980.13920574486428103No Hit
TAAAACGTTTTTTTGAAAGCTTTTAAAGT82420.13661987963460398No Hit
TCATTTAGGTGAATGTCTGCGGCCACGTC81180.13456444829819403No Hit
CAGCCATAAAATGCATCATTCTTTTTTGT75960.1259117454142747No Hit
ACACAGACTTCAGCAGTGAATAATGACTT75350.1249006058052343No Hit
ATGTGCTGAATGTTAATCCACCATCTACA74320.12319327171128086No Hit
GCCTCTTTCTGTAGTAGTTTTTTGATTTC72010.11936420204425907No Hit
GACACAATATGAAAAAATGTATATTCGTT69970.1159826859746814No Hit
CTGAAGACTACATCCAATGGACACCAAGC68840.11410959128908199No Hit
TCAGCGTTTGCAGATGAGAACAGCTCTCA67610.11207073601183665No Hit
CAGGTTTGCTTTGTTGTGGCCTGCAGTAA66950.11097671610697327No Hit
AGATGTCAGAGGCACTTCAATAATCACAC65920.10926938201301983No Hit
CGGATTCGCTTTTAGTCTCCTCTTAATAG64720.10728025491326826No Hit
GGATAAAATCCCTAGGAGGAGTTCGTTAA63770.10570552929263159No Hit
GGTCATCATGTCTGAAAAAAATAACTAAA62580.10373297825204462No Hit
TTTCATGAGACTTGAACCGGCGAACAGCA61820.10247319775553529No Hit
AAGCTCTGCCTATTACATCCAGAGCTGGT61050.10119684119986136No Hit
TGCGCGTTTTGAACCGCTGTCTGCACAAG60540.10035146218246693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC703.6379788E-1223.000443
AGCCGCG653.8198777E-1123.000448
GATCGGG352.2825217E-523.000441
CGACCTA1800.023.000445
GCCGGAC1000.023.000445
GACCCCG502.8916475E-823.000442
CCGTCGG2700.023.000443
CAGGCGA250.001993776823.000442
CGGGCGC352.2825217E-523.000444
CGTGTCG1350.023.000446
ACGTAGC1400.023.000441
TAGTGCC1350.023.000445
GACTCAC703.6379788E-1223.000447
CCCGCCG900.023.000442
TGCGTCG1800.023.000447
CGACGGG2950.023.0004393
GCGAACT302.1275344E-423.0004395
ACCGACG850.023.0004394
CGCGCGA402.4603087E-623.0004397
CGTCTAG302.1275344E-423.0004396
Produced by FastQC (version 0.11.5)